Introduction

Welcome to our comprehensive suite of custom molecular biology products and services tailored to meet your research needs. Our team of experts is dedicated to providing high-quality solutions to drive innovation in your molecular biology experiments.

  • Custom Primers & Oligonucleotides:
  • Custom DNA/RNA Synthesis:
  • Custom Cloning & Gene Synthesis:
  • Custom Molecular Probes & Recombinant Proteins:
  • Custom CRISPR/Cas9 Tools & Cell Lines:
  • Custom Assay Development:

Conclusion: Unlock the full potential of your molecular biology research with our customizable products and services. Whether you need custom primers, synthetic genes, or specialized assays, we’re here to support your scientific endeavors every step of the way. Contact us today to discuss your project requirements and discover how we can help advance your research goals.

DNA Oligo Synthesis

GenCefe provides high-quality DNA oligo synthesis and modification services, including standard DNA primers, modified DNA oligos, qPCR primers and probes, NGS primers and probes, etc. We have established corresponding production processes and quality control standards for different applications, such as scientific research and in vitro diagnostics.

Service Advantages：

High QualityStrict QCTechnical SupportCustomized Synthesis
Purity>95%,
molecular weight bias<0.05%,
batch-to-batch stability		ISO9001 certified, GMP-like clean room, customized QC itemsStandardized project management, professional pre-sales and after-sales servicesCustomized synthesis of various modified primers and probes, up to gram level

DNA Oligo Synthesis Services：

DNA Oligos for Molecular Biology ResearchDNA Oligos for Molecular Diagnostics
Standard primer
High-purity primer
Modified/Labeled prime		qPCR Primers/Probes
IVD qPCR primers
IVD qPCR probes
Forensic Diagnosis
STR fluorescent-labeled primers		NGS Primers
NGS adapter
NGS capture probes
NGS multiplex PCR primers
NGS blocker

DNA Oligo Modifications

GenCefe provides customized DNA oligo modification/labeling services, including more than 100 modifications such as biotin, digoxigenin, phosphate, thiol, sulfhydryl, amino, special bases, and a variety of fluorescent groups. 100% of the delivered modified probes/primers have passed mass spectrometry detection to ensure accuracy and purity, meeting the needs of scientific research and industrial-grade modified primers/probes.

Oligo Modification Position:

DNA Oligo Modification Case Study:

125 nt, Spacer18 modification, multiple Poly-T sequences, purity>95.58%, molecular weight bias<0.05%

DNA Modification Groups:

Rare BaseFluorescenceFluorescenceIntermediateOthers
dI
dU
m5C
m6A
2′ -O-Methyl base
RNA base
LNA		5′ Cy3
3′ Cy3
5′ Cy5
3′ Cy5
5′ FAM
3′ FAM
5′ HEX
5′ TET
5′-FITC
3′-FITC
5′ 6-JOE
3′ 6-JOE
5′ ROX
3′ ROX
5′ TAMRA
3′ TAMRA
3′ -HEX
3′ -TET
5′ Helix Flour555
3′ Helix Flour555
5′ Helix Flour575
3′ Helix Flour575
5′ Texas Red
3′ Texas Red
5′ Quasar 570
3′ Quasar 570
5′ Quasar 670
3′ Quasar 670		5′ Cy3.5
3′ Cy3.5
5′ Cy5.5NS
3′ Cy5.5NS
5′ Cy7NS
3′ Cy7NS
5’&3′ CAL Fluor Red 590
5’&3′ CAL Fluor Red 610
5’&3′ DABCYL
5’&3′ AMCA
5’&3′ DEAC
5’&3′ MCA
5′-VIC
5′-SIMA		Int NH2 dT
Int Biotin dT
Int Cy3 dT
Int Cy5 dT
Int TAMRA dT
Int ROX dT
Int FAM dT
Int HEX dT
Int Dig dT
Int CHCH dT
Int DBCO dT
Int Azide dT
Int BHQ1 dT
Int BHQ2 dT
Int HS-SH C6
Int Alexa Flour dT
Int Azobenzene
Int Ferrocene dT
dT- LC RED-610
dT- LC RED-640
dT- LC RED-705
dC-Fluorescein
dC-Tamra
dC-Amino
dC-Rox
dC-Digoxin
dC-FITC
dC-Texas Red		Azide
DBCO
Spacer9
Spacer(C12)
Spacer C3
Spacer C18
dSpacer
Inverted Base
5′ -Carboxy C10
5′ -Acridine
3′ ddC
Alexa Flour 647
Yakima Yellow Epoch
Pyrene
Phosphorothioate
S
Phosphorylation
5′ PHO
3′ PHO
Amino Linkers
5′ NH2 C6
3′ NH2 C7
5′ NH2 C12
ThiolQuenchers
3′ SH C3
5′ SH C6		3′ DABCYL
3′ TAMRA
3′ BHQ 1
3′ BHQ 2
3′ BHQ3
3′ -MGB
3′ eclipse
Biotin
5′ Biotin
5′ Biotin TEG
3′ Biotin
3′ Biotin TEG
Digoxigenin
5′ DIG
3′ DIG

Dual-Labeling Probes:

TAMRAECLIPSBHQ1BHQ3
5′ FAM-3′ TAMRA
5′ HEX-3′ TAMRA
5′ TET-3′ TAMRA
5′ JOE-3′ TAMRA
5′ VIC-3′ TAMRA		5′ FAM-3′ ECLIPS
5′ HEX-3′ ECLIPS
5′ TAMRA-3′ Eclipse
5′ ROX-3′ Eclipse
5′ TET-3′ Eclipse
5′ JOE-3′ Eclipse		5′ FAM-3′ BHQ1
5′ VIC-3′ BHQ1
5′ HEX-3′ BHQ1
5′ JOE-3′ BHQ1
5′ TET-3′ BHQ-1		5′ Quasar 670-3′ BHQ3
5′ CY5-3′ BHQ3
MGBDabcylBHQ2Others
5′ FAM-3′ MGB
5′ HEX-3′ MGB
5′ TET-3′ MGB
5′ JOE-3′ MGB
5′ ROX-3′ MGB
5′ Texas Red-3′ MGB
5′ Quasar 670-3′ MGB
5′ VIC-3′ MGB		5′ FAM-3′ DABCYL
5′ HEX-3′ DABCYL
5′ TET-3′ DABCYL
5′ JOE-3′ DABCYL
5′ TAMRA-3′ DABCYL
5′ Texas Red-3′ Dabcyl
5′ ROX-3′ Dabcyl
5′ CY3-3′ DABCYL
5′ Cy5-3′ DABCYL		5′ Cy5-3′ BHQ2
5′ Cy3-3′ BHQ2
5′ ROX-3′ BHQ2
5′ TET-3′ BHQ2
5′ JOE-3′ BHQ2
5′ HEX-3′ BHQ2
5′ TAMRA-3′ BHQ2
5′ Texas Red-3′ BHQ2
5′ Quasar 670-3′ BHQ2
5′ -FAM and 3′ -BHQ2		5′ Phos and 3′ FAM
5′ TAMRA and 3′ Phos
5′ C6-Biotin and 3′ Biotin
5′ C6-Biotin and 3′ Cy5
5′ C6-NH2 and 3′ C3-Fam
5′ C6-NH2 and 3′ Cy3
5’Cy5 and SS
5′ Cy5 and 3′ C7-NH2
5′ Cy5 and 3′ SH
5′ Dabcyl and 3′ C3-Fam
5′ DlG and 3′ Digoxin
5′ SH and 3′ Cy3
5′ Fam and 3′ C3-Fam
5′ TAMRA and 3′ C3-Fam